Qualidade da carne de frangos da linhagem Label Rouge suplementados com minerais biocomplexados e criados em sistema alternativo / [Meat quality of Label Rouge lineage chicken supplemented with biocomplexed minerals and reared in an alternative system]

Objetivou-se avaliar a influência da suplementação com minerais biocomplexados sobre a qualidade da carne de frangos da linhagem Label Rouge, de ambos os sexos, criados em sistema alternativo. O delineamento experimental foi inteiramente ao acaso (DIC), disposto em esquema fatorial (3x2), sendo três dietas (controle; 0,50ppm de selênio; 0,40ppm de cromo) e dois sexos. Para a estabilidade lipídica, foi utilizado o DIC, disposto em esquema fatorial (3x2x2), sendo três dietas, dois sexos e dois tempos de armazenamento (zero e 12 meses). Os parâmetros avaliados no peito e na coxa foram: pH final, cor (L* - luminosidade, a* - índice de vermelho, b* - índice de amarelo, C* - índice de saturação e h* - ângulo de tonalidade), perda de peso por cozimento (PPC), força de cisalhamento (FC) e estabilidade lipídica. Além disso, foram avaliadas a proporção das formas químicas da mioglobina da coxa e a quantificação do conteúdo de selênio no peito. Não houve efeito isolado da dieta sobre os parâmetros físicos e químicos do peito e da coxa e sobre a concentração de selênio no peito. As fêmeas apresentaram médias superiores de L* (57,57), b* (10,55) e C* (10,60) do peito; e os machos de L* (55,09) da coxa. Entre os machos, as aves alimentadas com a dieta controle e com cromo expressaram valores superiores de FC da coxa em relação às suplementadas com selênio; para o tratamento com cromo, os machos apresentaram média superior de FC da coxa e, em contrapartida, para o tratamento com selênio, as fêmeas manifestaram a maior média. As amostras submetidas ao período de 12 meses de armazenamento mostraram maiores valores de substâncias reativas ao ácido tiobarbitúrico (TBARS) em ambos os cortes. A suplementação com cromo e selênio biocomplexados não provocou alterações na qualidade da carne; as fêmeas demonstraram melhores atributos de qualidade da carne.(AU)

The objective of the present study was to evaluate the influence of the supplementation with biocomplexed minerals on the quality of the meat of label Rouge lineage chicken, of both genders, reared in an alternative system. The experimental design was completely randomized (CRD) in a factorial scheme (3x2), with three diets (control; 0.50ppm of selenium; 0.40ppm of chromium) and two genders. For lipid stability, the CRD arranged in a factorial scheme (3x2x2) was used, being three diets, two genders and two storage times (0 and 12 months). The parameters evaluated in the breast and in the thigh were: final pH, color (L* - luminosity, a* - red index, b* - yellow index, C* - saturation index and h* - tonality angle), weight loss per cooking (WLC), shear force (SF) and lipid stability. In addition, we evaluated the proportion of chemical forms of myoglobin of the thigh and the quantification of selenium content in the breast. There was no isolated effect of diet on the physical and chemical parameters of the breast and thigh and selenium concentration in the breast. Females had higher mean values of L* (57.57), b* (10.55) and C* (10.60) of the breast; and males of L* (55.09) of the thigh. Among males, poultry fed with the control diet and chromium showed higher SF values of the thigh than those supplemented with selenium; for the treatment with chromium, the males presented superior average of SF of the thigh and, in contrast, for the treatment with selenium, the females had the highest average. Samples submitted to the period of 12-month of storage showed higher values of substances reactive to thiobarbituric acid (TBARS) in both cuts. Supplementation with biocomplexed chromium and selenium did not cause changes in meat quality; females presented better attributes of meat quality.(AU)

El cromo-levadura y la ractopamina afectan el perfil de ácidos grasos y la calidad de la carne en cerdos / Chromium-yeast and ractopamine affect the profile of fatty acids and quality of meat in pigs

RESUMEN Los consumidores de cerdo demandan productos bajos en grasa y sin aditivos que afecten la salud humana. El objetivo de este trabajo fue evaluar el efecto del cromo-levadura o ractopamina adicionadas a la dieta de cerdos, sobre el rendimiento, las características de la canal y la calidad de la carne. Se distribuyeron 20 cerdos de manera aleatoria en cuatro tratamientos: sin adición de cromo-levadura, ni ractopamina (T1); 10ppm de ractopamina (T2); 0,2ppm de cromo-levadura (T3) y 0,4ppm de cromo- levadura (T4). Se determinó el espesor de la grasa dorsal al inicio, al intermedio y al final del estudio. El rendimiento en canal caliente y fría se estimó en el frigorífico. Muestras de la cabeza del lomo fueron tomadas para evaluar pH, resistencia al corte, goteo, temperatura, retención de agua y contenido de ácidos grasos. Se realizó un análisis descriptivo de los datos y de Kruskal-Wallis, para determinar el efecto del tratamiento sobre las variables medidas. Ninguna suplementación afectó la ganancia de peso, peso final, conversión alimenticia, consumo alimento, rendimiento en canal caliente, rendimiento canal fría y grasa dorsal (P>0,05). La resistencia al corte aumentó con la ractopamina, pero disminuyó con el cromo-levadura. Las pérdidas por goteo fueron significativas con 0,2ppm de cromo-levadura, pero no lo fueron con 0,4ppm de cromo-levadura o ractopamina. No se encontró efecto de la ractopamina sobre los ácidos grasos, pero sí del cromo-levadura. Tanto el cromo-levadura como la ractopamina modifican características de la carne, como la dureza y el perfil de ácidos grasos.

ABSTRACT The consumers of pork demand products low in fat and free of additives that affect the human health. The objective of this study was to evaluate the effect of chromium-yeast or ractopamine added to the diet of pigs on the performance, characteristics of the carcass and meat quality. Twenty pigs were randomized to four treatments: without the addition of chromium-yeast or ractopamine (T1); 10ppm of ractopamine (T2); 0.2ppm of chromium-yeast (T3) and 0.4ppm of chromium-yeast (T4). The backfat thickness was evaluated at the beginning, at the intermediate and at the end of the experiment. Hot carcass yield and cold carcass yield was evaluated in the slaughter house. Loin samples were taken to evaluate pH, shear force, drip loss, temperature, water retention and fatty acid composition. A descriptive analysis of the data and Kruskal-Wallis was carried out to determine the effect of the treatment on the measured variables. Any supplementation affected significantly weight gain, final weight, feed conversion, feed intake, hot carcass yield, cold carcass yield and backfat thinkness (P>0,05). Shear force increased with ractopamine, but decreased with chrome-yeast. Drip losses were significant at 0.2ppm chromium-yeast, but were not significant at 0.4ppm chromium-yeast or ractopamine. There was no effect of ractopamine on fatty acids, but with chromium-yeast was significant. Both chrome-yeast and ractopamine modify meat characteristics such as hardness and fatty acid profile.

Effect of castration and vitamin E supplementation on carcass and meat quality of Santa Inês lambs / Efecto de la castración y de la suplementación con vitamina E sobre la canal y la calidad de la carne de corderos Santa Inês / Efeito da Castração e da suplementação de vitamina E sobre a carcaça e qualidade da carne de ovinos Santa Inês

Abstract Background: Carcass and sheep meat quality are influenced by several factors, such as race, age, slaughter weight, type of diet, and dietary supplementation. Objective: To evaluate the effect of castration and vitamin E supplementation on performance, carcass characteristics and meat of lambs slaughtered at 45 kg. Methods: Thirty-two male lambs (16 castrated and 16 not castrated) were fed diets with or without the inclusion of vitamin E. The animals had an average age of 180 ± 20 days and a mean weight of 25.1 ± 4.6 kg. Performance was evaluated for 56 days, and the animals were slaughtered when reaching a weight of 45 kg. The experimental design was randomized blocks based on initial weights, and variables were assessed by a 2x2 factorial design (castrated and intact, supplemented or not with vitamin E, with a probability of 5%). Results: Higher proportion (p<0.05) of visceral fat was found in castrated animals (kidney: 2.45%, inguinal: 0.57% and omental: 4.96%) than in intact animals (kidney: 1.25%, inguinal 0.27%. and omental 3.12%). The aged meat samples showed no color differences for any of the treatments, but there were differences in shear force for steaks aged for 7 days, where the castrated animals showed an average of 2.64 kgf and intact animals showed an average 3.29 kgf. The animals supplemented with vitamin E had lower shear forces in non-aged samples. Conclusion: Castration increased deposition of visceral and fat coverage but did not influence the cut yields and total carcass yield. The animals supplemented with vitamin E had lower shear forces in non-aged samples.

Resumen Antecedentes: La calidad de la canal y de la carne de ovino puede ser influenciada por varios factores, como raza, edad, peso de sacrificio, tipo de dieta y suplementación. Objetivo: Evaluar el efecto de la castración y de la suplementación con vitamina E sobre el desempeño, la calidad de la canal y la carne de corderos Santa Inés sacrificados a los 45 kg de peso. Métodos: Fueron utilizados treinta y dos corderos: dieciséis castrados y dieciséis no castrados, alimentados con dietas con o sin inclusión de vitamina E. Los animales tenían una edad promedio de 180 ± 20 días y un peso promedio de 25,1 ± 4,6 kg. Fue evaluado el desempeño durante 56 días y, al llegar a los 45 kg de peso fueron sacrificados. El diseño experimental fue en bloques aleatorizados con base en el peso inicial, y las variables analizadas mediante un modelo factorial 2x2 (castrado o no castrado y suplementado o no con vitamina E, con una probabilidad del 5%). Resultado: Se encontró mayor proporción (p<0,05) de grasa visceral en los animales castrados (renal: 2,45%; inguinal: 0,57% y mesentérica: 4,96%) que en los no castrados (renal: 1,25%; inguinal 0,27% y mesentérica: 3,12%). Las muestras de carne maduradas no presentaron diferencias de color entre tratamientos, sin embargo hubo diferencias en la fuerza de corte para las carnes maduradas por 7 días, donde los animales castrados presentaron una media de 2,64 kgf y los no castrados de 3,29 kgf. La carne de los animales suplementados con vitamina E tuvo menor fuerza de corte en las muestras no maduradas. Conclusión: La castración aumentó la deposición de grasa visceral y de cobertura pero no influenció las características de rendimiento total de la canal y cortes. Los animales suplementados con vitamina E tuvieron menor fuerza de corte en las muestras no maduradas.

Resumo Antecedentes: A qualidade da carcaça e da carne ovina pode ser influenciada por vários fatores como raça, idade, peso de abate tipo de dieta e suplementação. Objetivo: Avaliar o efeito da castração e da suplementação de vitamina E sobre o desempenho e qualidade de carcaça e carne de cordeiros Santa Inês abatidos a 45 kg de peso. Métodos: Foram utilizados trinta e dois cordeiros, dezesseis castrados e dezesseis não castrados alimentados com dietas com ou sem inclusão de vitamina E. Os animais tinham idade média de 180 ± 20 dias e peso médio de 25,1 ± 4,6 kg. Foi avaliado o desempenho durante 56 dias, e ao chegarem aos 45 kg foi realizado o abate. O delineamento experimental foi em blocos ao acaso, com base no peso inicial, e as variáveis analisadas por modelo fatorial 2x2 (castrado ou não castrado e suplementado ou não com vitamina E, ao nível de 5%). Resultado: Foi encontrada maior proporção (p<0,05) das gorduras viscerais nos animais castrados (renal: 2,45%; inguinal: 0,57% e omental: 4,96%) do que nos animais não castrados (renal: 1,25%; inguinal 0,27% e omental: 3,12%). As amostras maturadas não apresentaram diferenças de cor entre os tratamentos, no entanto houve diferenças na força de cisalhamento para as carnes maturadas por 7 dias, na qual os animais castrados apresentaram média de 2,64 kgf e os não castrados de 3,29 kgf. Os animais suplementados com vitamina E apresentaram menores forças de cisalhamento nas amostras não maturadas. Conclusão: A castração aumentou a deposição de gorduras viscerais e de cobertura, mas não influenciou as características de rendimento de carcaça e cortes. Os animais suplementados com vitamina E tiveram menor força de cisalhamento nas amostras não maturadas.

Biomechanical Properties of the Modified Memory Alloy Internal Fixator for Separation of Pubic Symphysis / 医用生物力学

Objective To investigate biomechanical characteristics of the modified memory alloy internal fixator for separation of pubic symphysis. Methods The model of pubic symphysis separation injury was established based on 10 pelvic specimens. The control group was fixed with the dynamic compression plate after reduction, and the experimental group was fixed with the modified memory alloy internal fixator for separation of pubic symphysis after reduction. The biomechanical stability for two kinds of internal fixation was compared. Results There were no loosening and fracture of internal fixation in both groups. The displacement of pubic symphysis in horizontal, anterio-posterior and vertical direction in the experimental group was obviously reduced compared with the control group (P＜0.05). Conclusions Compared with the dynamic compression plate, the modified memory alloy internal fixator for separation of pubic symphysis shows better resistance to the tensile force against horizontal and anterio-posterior direction, as well as better resistance to the vertical shear force.

Technological and sensory characteristics of hamburgers added with chia seed as fat replacer / Características tecnológicas e sensoriais de hambúrgueres adicionados de semente de chia como substituta de gordura

ABSTRACT: In order to improve nutritional and functional quality, chicken burgers were made with different amounts of chia seed (0, 2, 4 and 8%) as pork fat replacers and evaluated for the microbiological, technological and sensorial characteristics. The proximate composition and the microbiological characteristics of raw burgers were in accordance with Brazilian legislation. Higher additions of chia increased the amount of dietary fiber (up to 1.46%) and carbohydrate in the products and reduced the fat content by up to 29%. The addition of chia seed reduced the a* values and increased the h* values of the raw burgers and reduced the tenderness and degree of lipid oxidation in the cooked burgers. Lower sensory taste scores and overall acceptance were conferred on products with higher chia addition; although, the degree of acceptance of the products was high. Results of this study highlighted the potential use of chia seed in the elaboration of low fat and enriched dietary fiber burgers.

RESUMO: No intuito de melhorar a qualidade nutricional e funcional, hambúrgueres de frango foram elaborados com diferentes quantidades de semente de chia (0, 2, 4 e 8%) em substituição ao toucinho e avaliados quanto às características microbiológicas, tecnológicas e sensoriais. A composição centesimal e as características microbiológicas dos hambúrgueres crus estavam de acordo com o preconizado pela legislação Brasileira. Maiores adições de chia aumentaram a quantidade de fibra dietética (até 1,46%) e de carboidrato nos produtos e reduziu os valores de gordura em até 29%. A adição de semente de chia reduziu os valores de a* e aumentou os valores de h* da cor dos hambúrgueres crus e reduziu a maciez e o grau de oxidação lipídica nos hambúrgueres cozidos. Menores notas sensoriais de sabor e a aceitação global foram conferidas aos produtos com maior adição de chia, embora o grau de aceitação dos produtos tenha sido elevado. Os resultados deste estudo destacam o potencial de uso da semente de chia na elaboração de hambúrgueres com baixo teor de gordura e enriquecidos de fibra dietética.

Meat quality of chicken of different crossings in alternative system / Qualidade da carne de frangos de diferentes cruzamentos em sistema alternativo

The purpose was to evaluate the effects of gender and of different genotypes of chickens on the physicochemical parameters and centesimal composition, related to the quality of meat. The design was completely randomized arranged in 5x2 factorial scheme, five genotypes (Índio Gigante - IG; New Hampshire - NHS; Gigante Negra de Jersey - GNJ; poultry from the crossing between the IG and NHS breeds - IG x NHS; and between the IG and GNJ breeds - IG x GNJ) and two genders, with five repetitions and each one represented by three poultry, totaling 150 animals, slaughtered at 105 days. The parameters evaluated on the breast and thigh were: centesimal composition (moisture, ether extract - EE, protein and ash), ultimate pH, color (L*- luminosity, a*- redness, b* -yellowness, C* - chroma index and h* - hue angle), weight loss by cooking (WLC) and shear force (SF). The IG genotype had the highest average of ultimate pH of the breast (6.03). The NHS and IG x NHS genotypes showed, respectively, higher average of L * (58.93) and a* (1.92) of the breast. The IG, IG x NHS and IG x GNJ showed the highest values of b* of the breast (12.53, 13.37 and 12.69, respectively). The IG poultry showed high average of SF of the breast and thigh (4.79 and 5.01kgf, respectively). The IG x NHS and IG x GNJ genotypes showed the lowest ultimate pH values of the thigh (6.13 and 6.02, respectively). The IG x GNJ genotype showed a high average of b* of the thigh (14.94) and the NHS had a high average of WCL (24.65%). The females showed higher averages of EE on the breast and ash on the thigh (1.03 and 1.11%, respectively). The IG x NHS and IG x GNJ poultry showed higher averages of EE of the breast (1.21 and 1.38 %, respectively). The poultry of IG breed and those from the crossing with NHS and GNJ presented meat quality characteristics more desirable by the consumer in relation to physicochemical parameters and centesimal composition, while genders showed no influence on these aspects.(AU)

Objetivou-se avaliar os efeitos de sexo e de diferentes genótipos de frangos sobre os parâmetros físico-químicos e a composição centesimal, relacionados à qualidade da carne. O delineamento foi inteiramente ao acaso, disposto em esquema fatorial (5x2), sendo cinco genótipos (Índio Gigante - IG; New Hampshire - NHS; Gigante Negra de Jersey - GNJ; aves oriundas do cruzamento entre as raças IG e NHS - IG x NHS; e entre as raças IG e GNJ - IG x GNJ) e dois sexos, com cinco repetições, sendo cada uma representada por três aves, totalizando 150 animais, abatidos aos 105 dias. Os parâmetros avaliados no peito e na coxa foram: composição centesimal (umidade, extrato etéreo - EE, proteína e cinzas), pH final, cor (L*- luminosidade, a*- teor de vermelho, b*- teor de amarelo, C*- índice de croma e h*- ângulo de tonalidade), perda de peso por cozimento (PPC) e força de cisalhamento (FC). O genótipo IG apresentou a maior média de pH final do peito (6,03). Os genótipos NHS e IG x NHS apresentaram, respectivamente, médias elevadas de L* (58,93) e a* (1,92) do peito. Os IG, IG x NHS e IG x GNJ apresentaram os maiores valores de b* do peito (12,53, 13,37 e 12,69, respectivamente). As aves IG apresentaram médias elevadas de FC do peito e da coxa (4,79 e 5,01Kgf, respectivamente). Os genótipos IG x NHS e IG x GNJ apresentaram os menores valores de pH final da coxa (6,13 e 6,02, respectivamente). O genótipo IG x GNJ apresentou média elevada de b* da coxa (14,94), e o NHS média elevada de PPC (24,65%). As fêmeas apresentaram maiores médias de EE no peito e de cinzas na coxa (1,03 e 1,11%, respectivamente). As aves IG x NHS e IG x GNJ apresentaram maiores médias de EE do peito (1,21 e 1,38%, respectivamente). As aves da raça IG e as oriundas de seu cruzamento com NHS e GNJ apresentaram características de qualidade de carne mais desejáveis pelo consumidor em relação aos parâmetros físico-químicos e de composição centesimal, enquanto o sexo não revelou influência sobre esses aspectos.(AU)

Influence of the amount of tooth surface preparation on the shear bond strength of zirconia cantilever single-retainer resin-bonded fixed partial denture

PURPOSE: Conventional resin-bonded fixed partial dentures (RBFPDs) are usually made with a two-retainer design. Unlike conventional RBFPDs, cantilever resin-bonded fixed partial dentures (Cantilever RBFPDs) are, for their part, made with a single-retainer design. The aim of this study was to compare the effect of tooth surface preparation on the bond strength of zirconia cantilever single-retainer RBFPDs. The objective is to evaluate the shear bond strength of these single-retainer RBFPDs bonded on 3 different amount of tooth surface preparation. MATERIALS AND METHODS: Thirty extracted bovine incisors were categorized to 3 groups (n=10), with different amounts of tooth surface preparations. Teeth were restored with single-retainer RBFPDs with different retainer surfaces: large retainer of 32 mm²; medium retainer of 22 mm²; no retainer and only a proximal connecting box of 12 mm². All RBFPDs were made of zirconia and were bonded using an adhesive system without adhesive capacity. Shear forces were applied to these restorations until debonding. RESULTS: Mean shear bond strength values for the groups I, II, and II were 2.39±0.53 MPa, 3.13±0.69 MPa, and 5.40±0.96 MPa, respectively. Statistical analyses were performed using a one-way ANOVA test with Bonferroni post-hoc test, at a significance level of 0.001. Failure modes were observed and showed a 100% adhesive fracture. CONCLUSION: It can be concluded that the preparation of large tooth surface preparation might be irrelevant. For zirconia single-retainer RBFPD, only the preparation of a proximal connecting box seems to be a reliable and minimally invasive approach. The differences are statistically significant.

Pathogenesis of foot blisters caused by weight-bearing march / 局解手术学杂志

Objective To analyze the related factors of foot blisters caused by long-distance weight-bearing march and to explore the pathogenesis of foot blisters to provide a useful way for the prevention and treatment.Methods After the 300 km march,counted the number who had accomplished the march,and then recorded the number of foot blisters,location of blisters,and abrasion of sole.Collected the data of gender,age,body mass index (BMI),hand dominance,and whether had bliters before the march through questionnaire.And the data were coded for analysis with SPSS 13.0 statistical package.Results The 7 cases who complete the whole march and 17 cases who already had foot blisters before the march were ruled out of the final statistics.Among the remaining 590 cases,there were 554 cases (93.9%)suffered from foot blisters.And there were 1 282 blisters in total,among which the plantar blisters occupied 98% (1 257 cases).The analysis showed that the incidence of foot blisters had no significant correlation with gender,left/right foot,hand dominance,BMI and age.The predilection sites of blisters were the second and third metatarsals (28.2%),the hallux (21.3%),the fifth metatarsal (18.1%),and the calcaneus (15.8%)of the left foot.The predilection sites of blisters were the second and third metatarsals (33.3%),the hallux (22.4%),the fifth metatarsal (18.6%),and the calcaneus (14.5%)of the right foot.In terms of the abrasion of sole,the lateral heel was worn out the most (34.6% on the left and 34.2% on the right).Conclusion The study confirmed that the incidence of foot blisters had no significant correla-tion with gender,left/right foot,hand dominance,BMI and age,which may be affected by the particularity of this march.Most of the foot blisters occurred in the planta,and the predilection sites of blisters were in accord with sites of of the abrasion of sole and the distribution of plantar shear force,which demonstrated the shear force is the most critical factor on the pathogenesis of foot blisters.

Efeito da temperatura ambiente e reutilização da cama na qualidade da carne de frangos de corte / Effect of environmental temperature and reuse of bed on broiler meat quality / Efecto de la temperatura ambiental y la reutilización de la cama sobre la calidad de la carne de pollos de engorde

O objetivo deste trabalho foi avaliar o efeito da temperatura do ambiente de criação e da reutilização da cama sobre o desempenho de frangos de corte, rendimento de carcaça e a qualidade físico-química da carne de peito produzida. Foram utilizados 900 pintinhos Cobb®, sendo 450 aves alojadas em uma câmara climatizada, munida de aquecedores e refrigeradores, e 450 aves em galpão convencional para criação de frangos. Em ambas as instalações as aves foram distribuídas entre a cama nova e reutilizada tratada com cal. Foram avaliados índices de desempenho, rendimento de carcaça e a qualidade físico-química do músculo Pectoralis major aos 42 dias de idade. O delineamento experimental utilizado foi inteiramente casualizado, em arranjo fatorial 2x2 (temperatura: ambiente e termoneutra; cama: nova e reutilizada) com cinco repetições cada. Não houve influência dos tratamentos no desempenho e rendimento de carcaça. Foi observada maior intensidade de vermelho e menor teor oxidação na carne de peito de aves criadas sob temperatura ambiente 2,74 e 0,180 mg TMP/kg, em relação às aves criadas em conforto térmico 2,20 e 0,230 mg TMP/kg, aos 42 dias de idade. Houve redução significativa da força de cisalhamento, que variou de 1,38 kgf/cm² na carne de aves criadas sobre cama nova a 1,86 kgf/cm² na carne de aves criadas sobre cama reutilizada, indicando que a utilização de cama de frango nova favorece a produção de carne mais macia.

The aim of this study was to evaluate the effect of environmental temperature and bed reuse on dressing percentage, breast quality and productive performance of broilers. A total of 900 Cobb® chicks were used, of which 450 were housed in a barn equipped with heaters and coolers while the rest (450 birds) were housed in a traditional shed for broiler production. In both facilities, birds were distributed either to a new or reused bed -the reused bed was previously treated with lime. Performance indices, dressing percentage and physicochemical quality of Pectoralis major muscle at 42 days of age were evaluated. The experimental design consisted in a completely randomized 2x2 factorial arrangement (temperature: thermo neutral or room temperature; bedding: new or reused bed) with five replicates per treatment. No effect of treatment on productive performance or dressing percentage was observed. Birds kept at room temperature had greater intensity of red color and lower breast oxidation (2.74 and 0.180 mg TMP/kg) compared to birds kept in the thermo-neutral environment at 42 days of age (TMP 2.20 and 0.230 mg/kg). Shear force was 1.38 kgf/cm² for the birds kept on new bed compared with 1.86 kgf/cm² for the animals kept on reused bed, indicating that the use of new bed favors meat tenderness.

El objetivo de este trabajo fue evaluar el efecto de la temperatura ambiental y de la reutilización de la cama sobre el desempeño productivo de pollos de engorde, rendimiento de canal y calidad fisicoquímica de la pechuga producida. Fueron utilizados 900 pollitos Cobb®, siendo 450 aves alojadas en un galpón equipado con calentadores y refrigeradores, y 450 aves alojadas en un galpón tradicional para la producción de pollos. En ambas instalaciones las aves fueron distribuidas entre la cama nueva y la cama reutilizada tratada anteriormente con cal. Fueron evaluados índices de desempeño, rendimiento de canal y calidad fisicoquímica del músculo Pectoralis major a los 42 días de edad. El diseño experimental fue completamente al azar con arreglo factorial 2x2 (temperatura: ambiente o termo neutra; camas: nueva o reutilizada) con cinco repeticiones cada uno. No hubo efecto de los tratamientos sobre el desempeño productivo y el rendimiento de canal. Se observó una mayor intensidad del color rojo y menor oxidación de la pechuga de aves mantenidas sobre temperatura ambiente 2,74 y 0,180 mg TMP/kg en relación a las aves mantenidas en ambiente termo neutro 2,20 y 0,230 mg TMP/kg a los 42 días de edad. Hubo una reducción significativa de la fuerza de corte, que vario de 1,38 kgf/cm² en la carne de aves mantenidas sobre cama nueva a 1,86 kgf/cm² en la carne de aves mantenidas sobre cama reutilizada, indicando que el uso de cama nueva favorece a la producción de carne más blanda.

Evaluación del grado de sellado marginal y resistencia adhesiva de restauraciones de resina compuesta con adhesivo convencional en dentición primaria y definitiva / Evaluation of microleakage and strength adhesive of composite resin restorations with total etch adhesive system in primary and permanent teeth

El presente estudio evaluó el grado de sellado marginal y resistencia adhesiva de restauraciones de resinas compuestas confeccionadas con un sistema adhesivo de grabado y lavado en dentición primaria y definitiva. Para realizar ambos test se utilizaron 20 piezas primarias y 20 piezas definitivas. En resistencia al cizallamiento, los dientes fueron seccionados longitudinalmente y se les adhirió un cilindro de resina compuesta, utilizando la técnica adhesiva de grabado y lavado para ambas denticiones. Posteriormente, fueron testeados en una máquina de ensayos universales Tinius Olsen, aplicando una fuerza de cizallamiento de 200 kg y a una velocidad de cabezal de 0,2 cm/min. Para el test de sellado marginal, se les realizó una preparación cavitaria clase V, por vestibular/palatino estandarizada. Luego se les confeccionaron restauraciones de resina compuesta con la técnica de grabado y lavado, y se sometieron al termociclado con azul de metileno al 2%; después fueron cortados y observados al microscopio óptico estereoscópico con aumento de lupa, para medir el porcentaje de microfiltración obtenido. El valor promedio de la resistencia adhesiva al cizallamiento para dentición primaria fue de 8,49 Mpa, y de 9,52 Mpa para dentición definitiva, no encontrándose diferencias estadísticamente significativas entre los grupos en estudio. En la investigación sobre el grado de sellado marginal se evaluó el porcentaje de microfiltración y tampoco se encontraron diferencias estadísticamente significativas, lo que señalaría que ambos tejidos son igualmente susceptibles de ser tratados adhesivamente mediante la técnica de hibridación.

The present study evaluated the degree of marginal seal and adhesive resistance of composite resin restorations prepared with an etch and rinse adhesive system in primary and definitive dentition. To perform both tests, 20 primary pieces and 20 permanent pieces were used. In the shear strength resistance, the teeth were sectioned longitudinally and were adhered to composite resin cylinder, using the etch and rinse technique for both dentitions. They were then tested on a Tinius Olsen universal testing machine, applying a shear force of 200 kg and a head speed of 0.2 cm/min. For the margin seal test, a class V cavity preparation of standardised vestibular/palatine was made. The composite resin restorations were made with the etch and rinse technique and were subjected to thermocycling with 2% methylene blue. Later, they were cut and observed using a stereoscopic optical microscope with loop magnification to measure the percentage of microfiltration obtained. The mean value of the adhesive resistance to shearing for primary dentition was 8.49 Mpa, and 9.52 Mpa for permanent teeth, with no statistically significant differences being found between the groups studied. In the test on the degree of margin seal the percentage microfiltration was measured, with no statistically significant differences being found either. The results showed that both tissues are equally susceptible to being treated adhesively using the hybrid technique.

Características de carcaça e qualidade de carne em linhagens da raça Nelore / Carcass and meat quality traits in lineages of Nellore breed

O objetivo deste estudo foi avaliar diferenças entre linhagens da raça Nelore para características de carcaça e qualidade de carne. Foram avaliadas treze linhagens da raça Nelore para as características de peso de carcaça quente, área de olho de lombo, espessura de gordura subcutânea, marmoreio e força de cisalhamento aos 7,14 e 21 dias de maturação. Para isso, foram utilizadas informações fenotípicas de 516 animais da raça Nelore e estimadas as diferenças esperadas na progênie para comparação entre as linhagens. Dentre os genearcas estudados, Golias obteve os melhores valores das diferenças esperadas na progênie para peso de carcaça quente (+1,20kg), área de olho de lombo (+0,88cm), marmoreio (+3,47un) e força de cisalhamento média (-0,09kg) e Akasamu para espessura de gordura subcutânea (+0,05mm). As diferenças entre linhagens da raça Nelore encontradas neste estudo podem ser utilizadas na escolha de touros para melhoria genética de características de carcaça e carne em rebanhos de gado de corte brasileiros.

The aim of this study was to evaluate the differences between lineages of Nellore breed for carcass and meat quality traits. Thirteen lineages of Nellore breed were evaluated from data of carcass and meat quality of 516 animals for estimating the expected progeny difference for hot carcass weight, rib eye area, fat thickness, marbling and shear force values at 7, 14 and 21 days of ageing. The founder Golias reached the better values of expected progeny difference for hot carcass weight (+1.20kg), rib eye area (+0.88cm), marbling (+3.47un) and average shear force (-0,09kg), and Akasamu the better value for fat thickness (+0.05mm). The differences between lineages of Nellore breed found in this study couldbe used to select sires for genetic improvement of carcass and meat quality traits in Brazilian beef cattle herds.

Genotype effect on carcass and meat quality of lambs finished in irrigated pastures in the semiarid Northeastern Brazil / Efeito do genótipo sobre características de carcaça e qualidade da carne de cordeiros terminados em pastagem irrigada no semiárido do Nordeste do Brasil

The aim of this study was to compare four genetic groups of sheep on the carcass and meat quality traits. Thirty-three contemporary and unrelated male lambs, all of single birth were used in the experiment, being thirteen from the Santa Inês (SI) breed, seven from the Brazilian Somali breed (BS), six from the Morada Nova (MN) breed and seven from the ½ Dorper - ½ Morada Nova (F1) crossbreed. The genotypes SI, BS and F1 presented similar performances in relation to hot and cold carcass weights, which values were 10.76±0.53kg and 10.46±0.52kg for SI, 9.20±0.73kg and 8.99±0.71kg for BS, and 9.35±0.73kg and 9.13±0.71kg for F1, respectively. The BS had a better hot carcass yield (47.10±0.88%) and cold carcass yield (46.00±0.87%). Better carcass conformation was observed in SI and F1 (2.73±0.12 and 2.50±0.17, respectively) while the BS presented a better finishing (3.29±0.20). The average for the rib eye area (REA) was 9.94±0.49cm², 8.66±0.67cm², 7.18±0.72cm² and 9.8±0.67cm², and for the carcass compactness index (CCI) it was 0.17±0.01kg/cm, 0.17±0.01kg/cm, 0.11±0.01kg/cm and 0.16±0,01kg/cm, for SI, SB, MN and F1, respectively. There were no significant differences between SI, BS and F1 regarding REA and CCI. Although, in general, the MN presented a relatively lower performance than the other genotypes, this breed had similar carcass yields and fat thickness when compared to SI and F1 and similar conformation and REA in comparison to the BS. Regarding meat quality, no differences were observed between genotypes, except for redness and cooking losses. It is concluded that no one group had a higher or lower performance in all traits analyzed. Moreover, for the management conditions employed in this study, there was evidence of greater specialization in meat production for genotypes SI, BS and F1 when compared to MN, although there are no substantial differences between the four groups regarding meat quality.

O objetivo deste estudo foi comparar quatro grupos genéticos de ovinos quanto às características de carcaça e de qualidade da carne. Trinta e três cordeiros, contemporâneos, nascidos de parto simples e sem relação de parentesco, foram utilizados no experimento, sendo 13 da raça Santa Inês (SI), sete da raça Somalis Brasileira (SB), seis da raça Morada Nova (MN) e sete ½ Dorper - ½ Morada Nova (F1). Os genótipos SI, SB e F1 apresentaram desempenhos similares quanto ao peso das carcaças quente e fria, cujos valores foram 10,76±0,53kg e 10,46±0,52kg para o SI, 9,20±0,73kg e 8,99±0,71kg para o SB, 9,35±0,73kg e 9,13±0,71kg para o F1, respectivamente. A raça SB apresentou melhor rendimento de carcaça quente (47,10 ±0,88%) e de carcaça fria (46,00±0,87%). Os genótipos SI e F1 apresentaram melhores conformações (2,73±0,12 e 2,50±0,17, respectivamente), enquanto o SB apresentou melhor acabamento (3,29±0,20). As médias para área de olho de lombo (AOL) foram 9,94±0,49cm², 8,66±0,67cm², 7,18±0,72cm² e 9,8±0,67cm² e para o índice de compacidade da carcaça (ICC) foram 0,17±0,01kg/cm, 0,17±0,01kg/cm, 0,11±0,01kg/cm e 0,16±0,01kg/cm, para SI, SB, MN e F1, respectivamente. Não houve diferença significativa entre os genótipos SI, SB e F1 quanto à AOL e ao ICC. Apesar de, no geral, a raça MN apresentar desempenho relativamente inferior aos demais genótipos quanto às características de carcaça analisadas, essa raça apresentou rendimentos de carcaça e espessura de gordura similar ao SI e F1 e conformação e AOL similar ao SB. Não houve um grupo superior ou inferior para todas as características analisadas. Por outro lado, para as condições de manejo empregadas neste estudo, evidenciou-se maior especialização para produção de carne dos genótipos SI, SB e F1 em relação ao grupo MN. Não houve diferenças importantes entre os diferentes genótipos quanto à qualidade da carne.

Atributos de qualidade da carne de paca (Agouti paca): perfil sensorial e força de cisalhamento / Quality attributes of paca meat (Agouti paca): sensory profile and shear force

Avaliaram-se as características sensoriais e determinou-se a força de cisalhamento de cortes de carne de paca (Agouti paca). As análises foram realizadas nos cortes desossados de paleta, lombo e pernil de nove pacas, preparados por cocção até a temperatura interna de 70ºC. A avaliação de aspecto, cor, sabor, odor e maciez foi realizada pela aplicação de teste afetivo a 146 provadores, utilizando-se escala hedônica, e a força de cisalhamento foi determinada pela técnica Warner Bratzler. Na avaliação sensorial, os cortes de paleta, lombo e pernil de paca mostraram diferença significativa (p<0,05) para os atributos de aspecto e cor. A paleta obteve pontuação mais baixa para o aspecto e mostrou coloração mais forte em relação aos demais cortes. Para a força de cisalhamento, não houve diferença (p>0,05) entre os cortes, que se mostraram igualmente macios. A carne de paca apresentou-se sensorialmente semelhante à carne suína e com boa aceitação pelos consumidores. O estudo evidenciou o potencial da paca como uma espécie silvestre para a produção comercial de carne para o mercado de carnes vermelhas ou exóticas.

Sensory characteristics and shear force of paca meat (Agouti paca) were assessed in this study. Analyses were performed in the bonelessshoulder,loin andhamobtained from nine paca carcassesprepared by cookinguntil reaching the internal temperatureof 70°C. The evaluation of flavor, aroma, color, appearance and tenderness was carried out by the application of an affective test using the hedonic scaleand a 146 consumer panel. Shear force was determined by the Warner-Bratzler technique. Shoulder, loin and ham had significant differences (p<0.05) in appearance and color. Shoulder presented the lowest score for appearance and showed darker color compared to the other cuts. Shear force values had no significant differences (p > 0.05) among the cuts, which were similarly tender. Paca meat was found to resemble pork meat in sensory evaluation and had good acceptance by consumers. This study showed the potential of paca (Agouti paca) asa wild species for meat production in the red or exotic meat market.

Development of shear force-induced intervertebral disc degeneration model / 医用生物力学

Objective To develop a shear force-induced intervertebral disc degeneration (IDD) in vivo animal model, and investigate the relationship between shear stress and IDD. Methods A total of 20 Japanese white rabbits were randomly divided into two groups. In loading group (n=10), shear force of 50 N was applied on the disc of L4/5 for 4 weeks by a custom-made external shear force loading device. In control group (n=10), the animals underwent a sham operation with the external loading device situated, but their discs remained unloaded. After 4 weeks, all the intervertebral discs of L4/5 were executed for the pathologic examination. Results The postoperative radiographic examination showed a perfect position of the loading device, and the operation process and implanted loading device had no effect on daily activities and diet of the experiment animals. The pathological examination showed an irregular arrangement of annulus fibrosus and a significant decrease of normal nucleus pulposus cells in loading group. Conclusions The new custom made device greatly reduced the wounds on animal vertebra and provided a reliable shear force. The development of in vivo animal model indicates that IDD can be induced by shear force, which is of significance to further study the relationship between loading and IDD.

Composição física da carcaça e características da carne de bovinos de corte terminados em confinamento com diferentes dietas / Physical composition of carcass and meat characteristics of beef cattle finished in feedlot with different diets

Avaliou-se o efeito de diferentes dietas sobre as características da carcaça e da carne de bovinos de corte em confinamento: dieta composta por silagem de milho e formulação fixa durante o período de confinamento (SMF); dieta composta por silagem de milho e formulação variável durante o período de confinamento (SMV); dieta composta por silagem de cana-de-açúcar e formulação fixa (SCF); dieta composta por silagem de cana-de-açúcar e formulação variável (SCV); SCV na metade inicial do confinamento e SMV no período final (SCV/SMV). O delineamento experimental foi inteiramente ao acaso, com cinco tratamentos e três repetições. Houve maior deposição de gordura renal-pélvica-inguinal (P=0,03), de gordura subcutânea (P=0,06) e de gordura na carcaça (P=0,06) nas dietas com silagem de milho (6,7kg; 6,2mm e 34,6 por cento) em relação àquelas com silagem de cana-de-açúcar (5,3kg; 4,8mm e 31,7 por cento). Houve maior (P=0,05) rendimento de contrafilé e de miolo de alcatra nas dietas com silagem de milho (10 por cento e 6,9 por cento) em relação às com silagem de cana-de-açúcar (9 por cento e 6,6 por cento). Não houve efeito das dietas sobre rendimento de cortes primários (P>0,1), perdas por cocção (P>0,3) e força de cisalhamento (P>0,1). O ajuste da formulação da dieta e a troca de silagem não influenciaram as características de carcaça e da carne. A utilização de silagem de cana-de-açúcar permitiu carcaças com acabamento satisfatório.

The effects of different diets on the carcass and meat characteristics of beef steers in feedlot were evaluated using the following: diet containing corn silage, with fixed formulation during the period of feedlot (CSF); diet containing corn silage, with variable formulation according to the phase of feedlot (CSV); diet containing sugar cane silage with fixed formulation (SCSF); diet containing sugar cane silage, with variable formulation (SCSV); CSV diet in initial half of feedlot and SCSV diet in final half (SCSV/CSV). A completely randomized experimental design with five treatments and three replicates (collective pens) was used. There was higher content of kidney-pelvic-inguinal fat (P=0.03), subcutaneous fat (P=0.06), and fat tissue (P=0.06) deposition in animals fed diets containing corn silage (6.7kg, 6.2mm, and 34.6 percent) as compared to those fed sugar cane silage (5.3kg, 4.8mm, and 31.7 percent). A higher (P=0.05) yield of strip loin and rostbiff ( percent of hindquarter) were measured in carcasses from animals fed diets containing corn silage (10 percent and 6.9 percent) than in carcasses from animals fed diets containing sugar cane silage (9 percent and 6.6 percent). No effects of the diets on yield of primary meat cuts of the carcass (P>0.1), cooking losses (P>0.3), and shear force (P>0.1), with averages of 23.4 percent and 4.4kgf/mIII. The adjustment of the diet formulation as the period of confinement and the exchange of silage did not affect the characteristics of carcass and meat. The use of sugar cane silage allowed carcasses with good finishing.

Espessura da lâmina de cisalhamento na avaliação instrumental da textura da carne / Shear blade thickness in the instrumental evaluation of meat texture

A padronização da textura é um dos principais problemas relacionados à qualidade da carne bovina. Por isso, a avaliação objetiva da maciez, por métodos mecânicos, vem sendo estudada há muitos anos. Para que a avaliação instrumental da textura seja uma ferramenta efetiva nos estudos envolvendo a maciez da carne, é necessário minimizar as causas de variação envolvidas na análise. Atualmente, a força de cisalhamento Warner-Bratzler é o método mais amplamente empregado para essa finalidade. O protocolo padrão dessa técnica analítica preconiza a utilização de uma lâmina de cisalhamento de 1,016mm de espessura. Neste trabalho, foram comparados dados de textura de carne bovina obtidos com a lâmina de cisalhamento padrão Warner-Bratzler, de 1,016mm de espessura, e uma lâmina mais grossa, de 3,05mm de espessura. Após o abate, amostras de músculo Longissimus dorsi, semelhantes em relação aos teores de umidade, lipídeos e proteínas, foram submetidas a duas diferentes temperaturas de resfriamento, atingindo diferentes velocidades de queda de temperatura e, consequentemente, diferentes graus de contração do tecido muscular, o que foi confirmado pela determinação do comprimento dos sarcômeros. Os resultados demonstraram uma maior sensibilidade da lâmina padrão Warner-Bratzler na detecção de variações da textura da carne, em função da velocidade de resfriamento.

Standardization of texture is one of the main problems related to beef quality. Because of that, objective evaluation of tenderness performed by mechanic methodologies has been studied for years. In order to count on instrumental texture evaluation as a valuable tool in meat tenderness studies, it is necessary to minimize the causes of variation that might be involved in the trial. Nowadays, Warner-Bratzler shear force is widely used for meat tenderness determination. The standard protocol of this methodology establishes the utilization of shear blade 1.016mm tick. The aim of this study was to compare beef texture data provided by Warner-Bratzler standard shear blade, 1.016mm thick, with those provided by a thicker one, 3.05mm thick. After slaughtering, Longissimus dorsi muscle samples with similar contents of moisture, lipids and proteins were subjected to two different cooling temperatures,reaching different rates of temperature drop and hence different muscle contraction extents, which were confirmed by the determination of sarcomere length. Results indicated the superior sensibility of the Warner-Bratzler standard shear blade to detect variations in meat texture due to cooling velocity.

Coloração da gordura e qualidade da carne de ovelhas de descarte abatidas em distintos estágios fisiológicos / Fat coloring and meat quality of culling ewes slaughtered at distinct physiological stages

Avaliaram-se a qualidade física do músculo Longissimus lumborum de ovelhas de descarte da raça Santa Inês, abatidas em diferentes estágios fisiológicos, a cor da gordura subcutânea desse músculo e as possíveis correlações entre as variáveis de qualidade da carne. Utilizaram-se 21 ovelhas, distribuídas nos seguintes tratamentos: T1= ovelhas que permaneceram por 60 dias em lactação com seus respectivos cordeiros e abatidas um dia após o desmame das crias; T2= ovelhas que permaneceram por 60 dias em lactação com seus respectivos cordeiros e mais um período aproximado de 30 dias sem os cordeiros e posteriormente abatidas; e T3= ovelhas que não pariram durante o ano. A cor da carne e da gordura subcutânea não foram influenciadas pelo estágios fisiológico dos animais no momento do abate, com exceção da luminosidade da carne (T1= 41,13; T2= 37,56 e T3= 38,12 L*). A qualidade física da carne apresentou pouca variação entre os tratamentos, porém a força de cisalhamento (T1= 4,08; T2=2,86 e T3=2,35 kgf/cm²) e as perdas por cocção (T1=31,97; T2=35,70 e T3= 31,91 por cento) da carne diferiram entre os tratamentos. Não se observou alta correlação entre a maioria das características avaliadas.

The physical quality of the Longissimus lumborum muscle of Santa Inês culled ewes slaughtered at different physiological stages was evaluated as well as the color of the subcutaneous fat of the same muscle, and the correlations between meat quality traits. Twenty-one ewes were randomly allotted in the following treatments: T1= ewes which remained in lactation for 60 days with their respective lambs and slaughtered one day after weaning of the lambs; T2= ewes which remained in lactation for 60 days with their respective lambs, followed by an additional period of 30 days without the lambs and slaughtered afterwards; and T3= non pregnant ewes. The colors of meat and subcutaneous fat were not influenced by the physiological stage of the animals at the moment of slaughtering, except meat luminosity (T1= 41.13; T2= 37.56 and T3= 38.12 L*) . The physical quality of the meat presented little variation between treatments; however, shear force (T1= 4.08; T2=2.86 and T3=2.35 kgf/cm2) and losses by cooking (T1=31.97; T2=35.70 and T3= 31.91 percent) were affected by treatments. No high correlation between most of the evaluated traits was observed.

Carcass quality of feedlot finished steers fed yeast, monensin, and the association of both additives / Qualidade de carcaça de novilhos terminados com dietas contendo levedura, monensina e associação de ambos aditivos

To evaluate the effects of the supplementation of feed additives on carcass quality in beef cattle, 72 Nellore steers (339.5kg, 20-month old) were feedlot finished and fed for 91 days one of the following diets: 1) control with no additives; or added of 2) live yeast culture; 3) monensin; or 4) the association of both additives. After slaughter, renal, pelvic, and inguinal fat and hot carcass weights were recorded and carcass was split into muscle, bone, and trimmable fat. Carcass Longissimus muscle area and subcutaneous fat thickness at the 12th rib were measured and steaks of Longisimus muscle were taken to determine meat color, shear force, drip, and cooking losses. Yeast increased carcass dressing percentage but there were no effects on hot carcass weight, Longissimus area, subcutaneous fat thickness, percentage and weight of retail cut yield and trimmings. Feed additives had no effect on carcass pH, meat color, fat content, shear force, and drip losses. Supplementation of yeast, monensin or the association of both additives had no important effects on carcass traits and on meat quality of feedlot finished steers.

Avaliaram-se os efeitos da suplementação de aditivos alimentares sobre a qualidade de carcaça em bovinos de corte. Usaram-se 72 novilhos Nelore com média de peso de 339,5kg e 20 meses de idade, terminados em confinamento e alimentados por 91 dias com uma das quatro dietas: 1) dieta controle sem aditivos, ou com a adição de 2) leveduras vivas, 3) monensina ou 4) associação entre ambos aditivos. Após o abate, os pesos da gordura renal, pélvica e inguinal e da carcaça foram medidos e a carcaça dividida em músculos, ossos e aparas. Foram mensurados a área de olho de lombo e a espessura de gordura subcutânea sobre o músculo Longissimus na região da 12ª costela e foram obtidos bifes para a determinação da cor, força de cisalhamento e perdas por cocção e cozimento da carne. A levedura aumentou o rendimento de carcaça, mas não houve efeito dos tratamentos sobre o peso de carcaça, porção comestível e aparas. Os aditivos não influenciaram o pH da carcaça, a cor, a gordura intramuscular, a força de cisalhamento e as perdas por exsudação da carne. A suplementação, com levedura e com monensina em associação ou separadamente, não teve efeito importante sobre a qualidade da carcaça em novilhos terminados em confinamento.

MECHANICAL STRESS IN KNEE JOINT DURING RUNNING AT VARIOUS SPEEDS AND STEP LENGTHS / 体力科学

In order to give more effective instruction for running in sports medicine, the mechanical stresses in the knee joint during running at various speeds and step lengths were investigated.<BR>The subjects were five male sprinters. Running conditions were as follows : 1) running at four speeds (2.5 m/s, 4.5 m/s, 6.5 m/s and maximum running speed) with natural step lengths, 2) run-ning with three different step lengths (1.0 m, 1.5m and preferred step length) at 4.5 m/s running speed, and 3) running at maximum speed using four different step lengths (1.0 m, 1.5m 2.5m and preferred step length) . Running movements were recorded using a high speed video camera. And ground reaction forces were also measured by a force platform. The compressive force and shear force in the tibiofemoral joint were computed from the results of two dimensional motion analysis. That is, the external force caused by ground reaction forces, the internal force produced by the mus-cle to develop joint torque and total force (external+internal force) were computed for both com-pressive and shear forces.<BR>The total compressive force that affects the meniscus and articular cartilage in the tibiofemoral joint depended on the magnitude of internal force. The total compressive force increased with running speed and step length. Therefore, caution should be employed in changing running speed and step length for regulating the magnitude of total compressive force on the tibiofemoral joint. On the other hand, the total shear force that caused traction stress in the posterior cruciate ligament depended on the magnitude of external force. The posterior shear force was generated during the foot contact period, and increased with step length. As for total shear force in the tibiofemoral joint, care must be taken to regulate step length.